LOCUS       40924_13325        4051 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCRB1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Tsuchida Y, Kimura S, Suzuki N, Inui M, Yukawa H.
  TITLE     Characterization of a 24-kb plasmid pCGR2 newly isolated from 
            Corynebacterium glutamicum
  JOURNAL   Appl. Microbiol. Biotechnol. 87 (5), 1855-1866 (2010)
  PUBMED    20552356
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Inui M, Suzuki N, Nakata K, Yukawa H.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2008) Contact:Masayuki Inui Research Institute of 
            Innovative Technology for the Earth, Molecular Microbiology and 
            Biotechnology Group; 9-2 kizugawadai, kizugawa, kyoto 619-0292, 
            Japan URL :http://www.rite.or.jp/
REFERENCE   3  (bases 1 to 4051)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4051)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2010"; volume: "87"; issue: "5"; 
            pages: "1855-1866"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JUL-2008) Contact:Masayuki Inui Research Institute of Innovative
            Technology for the Earth, Molecular Microbiology and Biotechnology 
            Group; 9-2 kizugawadai, kizugawa, kyoto 619-0292, Japan URL 
            :http://www.rite.or.jp/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        101..203
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             204..860
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     protein_bind    1071..1092
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1107..1137
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1145..1161
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1169..1185
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1198..1254
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1255..1271)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1597..2185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2237..4051
                     /note="derived from Brevibacterium lactofermentum cryptric 
                     plasmid pBL1"
     CDS             2760..3965
                     /codon_start=1
                     /gene="rep"
                     /product="replicase"
                     /label=rep
                     /protein_id="BAG54977.1"
                     /translation="MYKITNSKALAGCHRWRRDEAVAVSWSSNGASQFEGLQNSHSRWG
                     SPLAELEVMGERRIELAIATKNHLAAGGALMMFVGTVRHNRSQSFAQVEAGIKTAYSSM
                     VKTSQWKKERARYGVEHTYSDYEVTDSWANGWHLHRNMLLFLDRPLSDDELKAFEDSMF
                     SRWSAGVVKAGMDAPLREHGVKLDQVSTWGGDAAKMATYLAKGMSQELTGSATKTASKG
                     SYTPFQMLDMLADQSDAGEDMDAVLVARWREYEVGSKNLRSSWSRGAKRALGIDYIDAD
                     VRREMEEELYKLAGLEAPERVESTRVAVALVKPDDWKLIQSDFAVRQYVLDCVDKAKDV
                     AAAQRVANEVLASLGVDSTPCMIVMDDVDLDAVLPTHGDATKRDLNAAVFAGNEQTILR
                     TH"
     gene            2760..3965
                     /gene="rep"
                     /label=rep
ORIGIN
        1 atctccgtcg aacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
       61 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      121 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      181 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      241 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      301 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      361 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      421 ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      481 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      541 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      601 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      661 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      721 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      781 atgccgtctg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      841 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      901 gtgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcag cttggcccag
      961 tgccaagctc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     1021 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     1081 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     1141 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccgaa
     1201 ttcgagctcg gtacccgggg atcctctaga gtcgacctgc aggcatgcaa gcttactggc
     1261 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     1321 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     1381 ccaacagttg cgcagcctga atggcgaatg agcttcttcc gcttcctcgc tcactgactc
     1441 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1501 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1561 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1621 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1681 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1741 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     1801 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1861 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1921 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1981 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     2041 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2101 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2161 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2221 tcagtggaac gataacacat gcagtcatgt cgtgctaatg tgtaaaacat gtacatgcag
     2281 attgctgggg gtgcaggggg cggagccacc ctgtccatgc ggggtgtggg gcttgccccg
     2341 ccggtacaga cagtgagcac cggggcacct agtcgcggat acccccccta ggtatcggac
     2401 acgtaaccct cccatgtcga tgcaaatctt taacattgag tacgggtaag ctggcacgca
     2461 tagccaagct aggcggccac caaacaccac taaaaattaa tagtccctag acaagacaaa
     2521 cccccgtgcg agctaccaac tcatatgcac gggggccaca taacccgaag gggtttcaat
     2581 tgacaaccat agcactagct aagacaacgg gcacaacacc cgcacaaact cgcactgcgc
     2641 aaccccgcac aacatcgggt ctaggtaaca ctgaaataga agtgaacacc tctaaggaac
     2701 cgcaggtcaa tgagggttct aaggtcactc gcgctagggc gtggcgtagg caaaacgtca
     2761 tgtacaagat caccaatagt aaggctctgg cggggtgcca taggtggcgc agggacgaag
     2821 ctgttgcggt gtcctggtcg tctaacggtg cttcgcagtt tgagggtctg caaaactctc
     2881 actctcgctg ggggtcacct ctggctgaat tggaagtcat gggcgaacgc cgcattgagc
     2941 tggctattgc tactaagaat cacttggcgg cgggtggcgc gctcatgatg tttgtgggca
     3001 ctgttcgaca caaccgctca cagtcatttg cgcaggttga agcgggtatt aagactgcgt
     3061 actcttcgat ggtgaaaaca tctcagtgga agaaagaacg tgcacggtac ggggtggagc
     3121 acacctatag tgactatgag gtcacagact cttgggcgaa cggttggcac ttgcaccgca
     3181 acatgctgtt gttcttggat cgtccactgt ctgacgatga actcaaggcg tttgaggatt
     3241 ccatgttttc ccgctggtct gctggtgtgg ttaaggccgg tatggacgcg ccactgcgtg
     3301 agcacggggt caaacttgat caggtgtcta cctggggtgg agacgctgcg aaaatggcaa
     3361 cctacctcgc taagggcatg tctcaggaac tgactggctc cgctactaaa accgcgtcta
     3421 aggggtcgta cacgccgttt cagatgttgg atatgttggc cgatcaaagc gacgccggcg
     3481 aggatatgga cgctgttttg gtggctcggt ggcgtgagta tgaggttggt tctaaaaacc
     3541 tgcgttcgtc ctggtcacgt ggggctaagc gtgctttggg cattgattac atagacgctg
     3601 atgtacgtcg tgaaatggaa gaagaactgt acaagctcgc cggtctggaa gcaccggaac
     3661 gggtcgaatc aacccgcgtt gctgttgctt tggtgaagcc cgatgattgg aaactgattc
     3721 agtctgattt cgcggttagg cagtacgttc tagattgcgt ggataaggct aaggacgtgg
     3781 ccgctgcgca acgtgtcgct aatgaggtgc tggcaagtct gggtgtggat tccaccccgt
     3841 gcatgatcgt tatggatgat gtggacttgg acgcggttct gcctactcat ggggacgcta
     3901 ctaagcgtga tctgaatgcg gcggtgttcg cgggtaatga gcagactatt cttcgcaccc
     3961 actaaaagcg gcataaaccc cgttcgatat tttgtgcgat gaatttatgg tcaatgtcgc
     4021 gggggcaaac tatgatgggt cttgttgtgt t
//
